Targeted metabolomics applied to mitochondrial disease

Juan Manuel Chao de la Barca, Lecturer - Hospital Practitioner

(JMChaoDeLaBarca @ chu-angers.fr)

JMChaoDeLaBarca @ chu-angers.fr (JMChaoDeLaBarca @ chu-angers.fr)

Keywords : Metabolomics

State of the art and objectives

Targeted metabolomics applied to mitochondrial disease using different experimental models (cell cultures, animal models) and to sexual dimorphism

Targeted metabolomics applied to other non-mitochondrial disease (IUGR, glaucoma, hypertension, reproductive medicine, etc.) in the framework of clinical research

Main results from the last 5 years

• Stress of the endoplasmic reticulum is involved in the physiopathology of Leber Hereditary Optic Neuropathy
• Metabolomics signature associated with ovarian failure
• Metabolomics signature of the optic nerve associated with dominant optic atrophy
• Metabolomics signature of the retina associated with light damage
• Plasma metabolomics signature associated with heart preconditioning

People involved

Stéphanie CHUPIN (engineer/technician)

Main publications and patents from the 5 last years

• Chao de la Barca JM, Simard G, Amati-Bonneau P, Safiedeen Z, Prunier-Mirebeau D, Chupin S, Gadras C, Tessier L, Gueguen N, Chevrollier A, Desquiret-Dumas V, Ferré M, Bris C, Kouassi Nzoughet J, Bocca C, Leruez S, Verny C, Miléa D, Bonneau D, Lenaers G, Martinez MC, Procaccio V, Reynier P. The metabolomic signature of Leber's hereditary optic neuropathy reveals endoplasmic reticulum stress. Brain. 2016;139:2864-2876. (IF 2017: 10,84)
• Chao de la Barca JM, Bakhta O, Kalakech H, Simard G, Tamareille S, Catros V, Callebert J, Gadras C, Tessier L, Reynier P, Prunier F, Mirebeau-Prunier D. Metabolic signature of remote ischemic preconditioning involving a cocktail of amino acids and biogenic amines. J Am Heart Assoc. 2016;5. (IF 2017: 4,45)
• Chao de la Barca JM, Simard G, Sarzi E, Chaumette T, Rousseau G, Chupin S, Gadras C, Tessier L, Ferré M, Chevrollier A, Desquiret-Dumas V, Gueguen N, Leruez S, Verny C, Miléa D, Bonneau D, Amati-Bonneau P, Procaccio V, Hamel C, Lenaers G, Reynier P, Prunier-Mirebeau D. Targeted metabolomics reveals early dominant optic atrophy signature in optic nerves of Opa1delTTAG/+ mice. Invest Ophthalmol Vis Sci. 2017;58:812-820. (IF 2017: 3,388)(https://iovs.arvojournals.org/article.aspx?articleid=2601247)
• Chao de la Barca JM, Huang NT, Jiao H, Tessier L, Gadras C, Simard G, Natoli R, Tcherkez G, Reynier P, Valter K. Retinal metabolic events in preconditioning light stress as revealed by wide-spectrum targeted metabolomics. Metabolomics. 2017;13:22. (IF 2017: 3,511)(https://link.springer.com/article/10.1007/s11306-016-1156-9)
• Chao de la Barca JM, Boueilh T, Simard G, Boucret L, Ferré-L'Hotellier V, Tessier L, Gadras C, Bouet PE, Descamps P, Procaccio V, Reynier P, May-Panloup P. Targeted metabolomics reveals reduced levels of polyunsaturated choline plasmalogens and a smaller dimethylarginine/arginine ratio in the follicular fluid of patients with a diminished ovarian reserve. Hum Reprod. 2017;32:2269-2278. (IF 2016: 5,02)

Collaborations

 Australian National University

Acknowledgements for the financial supports

• OUVRIR LES YEUX
• Berthe Fouassier (Fondation de France)