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Mitochondrial and Cardiovascular Physiopathology | UMR Inserm U1083 - CNRS 6015

Separated by coma

Ion channels in vascular function

Christian Legros, Professor

 

 

christian.legros@univ-angers.fr

 

 

 

 

People involved in the project

  • Christian Legros, Ph.D. (Professor)
  • César Mattei, Ph.D. (Associate-Professor)
  • Hélène Tricoire, Ph.D. (Associate-Professor)
  • Claire Legendre, Ph.D (Associate-Professor)
  • Florian Beignon (Ph.D. Student)
  • Joohee Park (Ph.D. Student)
  • Léa Réthoré (Ph.D. Student)
  • Christina Sahyoun (Ph.D. Student)
  • Jennifer Bourreau (Technician INSERM)
  • Cyril Lecorre (AJT. UA)

 

State of the art and objectives


Our group is interested in elucidating the role of voltage-gated sodium channels in the vascular function. We are currently working on different aspects of the cellular and molecular pharmacology of voltage-gated sodium channels (alkaloids, scorpion toxins…), the cell signalling they induce – particularly regarding the intracellular Ca2+ homeostasis – and their role in different cell types (endocrine cells, vascular endothelial cells…). Some of our projects also involve the pharmacological and physiological implication of GABA receptors and nicotinic acetylcholine receptors as key players of the vascular function and the regulation of the arterial blood pressure.

Project supported by

  • Région Pays de la Loire (pari scientifique SODIVASC 2017-2020, bourses de thèse Joohee Park et Florian Beignon)
  • DGA/MinDef (bourse de thèse 2018-2021 – Florian Beignon)
  • MESR (bourse de thèse 2019-2022 – Léa Rethoré)
  • ANR (projet TempoMito 2022-2025 – César Mattei)
  • PHC Cèdre (Liban - Christian Legros)

Collaborations

  • Nathalie Guérineau (IGF Montpellier)
  • Arnaud Monteil (IGF Montpellier)
  • Antoine Taly (Institute of Physico-Chemical Biology, CNRS UPR9080, Paris)
  • Marc Verleye (Biocodex, Gentilly)
  • Benjamin Marie (Muséum National d’Histoire Naurelle)
  • Mireille Chinain (Institut Louis Mallardé, Polynésie française)
  • Jordi Molgó (CNRS-CEA Saclay)
  • Nathalie Arnich (ANSES Maisons-Alfort)
  • Andrea Bourdelais (Level 1 Fasteners, Wilmington, USA)
  • Ziad Fajloun (Université Libanaise)
  • Céline Marionneau et Michel Dewaard (Institut du Thorax)

Découverte de nouveaux alcaloïdes de plantes possédant des potentiels thérapeutiques.

Review articles:

  • Shaymaa Khazaal, Julien Harb, Mohamad Rima, Cédric Annweiler, Yingliang Wu, Zhijian Cao, Ziad Abi Khattar, Christian Legros, Hervé Kovacic, Ziad Fajloun and Jean-Marc Sabatier. The Pathophysiology of Long COVID throughout the Renin-Angiotensin System.  Molecules 2022, 27(9), 2903; https://doi.org/10.3390/molecules27092903.
  • Mackieh R, Abou-Nader R, Wehbe R, Mattei C, Legros C, Fajloun Z, Sabatier JM. (2021). Voltage-Gated Sodium Channels: A Prominent Target of Marine Toxins. Mar Drugs. 19(10): 562.
  • Frangieh J, Rima M, Fajloun Z, Henrion D, Sabatier JM, Legros C, Mattei C. (2021). Snake Venom Components: Tools and Cures to Target Cardiovascular Diseases. Molecules. 26(8): 2223.
  • Colas S, Marie B, Lance E, Quiblier C, Tricoire-Leignel H, Mattei C. (2021). Anatoxin-a: Overview on a harmful cyanobacterial neurotoxin from the environmental scale to the molecular target. Environ Res. 193: 110590.
  • Delcourt N, Lagrange E, Abadie E, Fessard V, Frémy JM, Vernoux JP, Peyrat MB, Maignien T, Arnich N, Molgó J, Mattei C (2019). Pinnatoxins' Deleterious Effects on Cholinergic Networks: From Experimental Models to Human Health. Mar Drugs. 17(7): 425.
  •  Mattei C (2018). Tetrodotoxin, a Candidate Drug for Nav1.1-Induced Mechanical Pain? Mar Drugs. 16(2):72.
  •  Delcourt N, Claudepierre T, Maignien T, Arnich N, Mattei C (2017). Cellular and Molecular Aspects of the β-N-Methylamino-l-alanine (BMAA) Mode of Action within the Neurodegenerative Pathway: Facts and Controversy. Toxins (Basel). 10(1): 6.

Research articles:

  • César Mattei, Hélène Tricoire-Leignel, Christian Legros, Richard J. Lewis and Jordi Molgó. Editorial: Pharmacological Aspects of Ligand-Gated Ion Channels as Targets of Natural and Synthetic Agents. Front. Neurosci., 08 April 2022, Sec.Neuropharmacology https://doi.org/10.3389/fnins.2022.895299.
  • Jacinthe Frangieh,Claire Legendre, Dimitri Bréard, Pascal Richomme, Daniel Henrion, Ziad Fajloun,César Mattei, Anne-Marie Le Ray and Christian Legros. Oxostephanine, Thalmiculine, and Thaliphyline—Three Isoquinoleine Alkaloids That Inhibit L-Type Voltage-Gated Ca2+ Channels. Future Pharmacol. 2022, 2(3), 238-255; https://doi.org/10.3390/futurepharmacol2030016.
  • Quentin Coquerel, Claire Legendre ,Jacinthe Frangieh, Stephan De Waard, Jérôme Montnach, Leos Cmarko, Joseph Khoury, Charifat Said Hassane, Dimitri Bréard, Benjamin Siegler, Ziad Fajloun, Harold De Pomyers, Kamel Mabrouk, Norbert Weiss, Daniel Henrion, Pascal Richomme, César Mattei, Michel De Waard, Anne-Marie Le Ray,  and Christian Legros. Screening an In-House Isoquinoline Alkaloids Library for New Blockers of Voltage-Gated Na+ Channels Using Voltage Sensor Fluorescent Probes: Hits and Biases. Molecules 2022, 27(13), 4133; https://doi.org/10.3390/molecules27134133.
  • Christina Sahyoun, Wojciech Krezel, César Mattei, Jean-Marc Sabatier, Christian Legros, Ziad Fajloun and Mohamad Rima. Neuro- and Cardiovascular Activities of Montivipera bornmuelleri Snake Venom. Biology 2022, 11(6), 888; https://doi.org/10.3390/biology11060888.
  • Réthoré L, Park J, Montnach J, Nicolas S, Khoury J, Le Seac'h E, Mabrouk K, De Pomyers H, Tricoire-Leignel H, Mattei C, Henrion D, Fajloun Z, De Waard M, Legendre C, Legros C. Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells. Int J Mol Sci. 2022 Jan 13;23(2):827. doi: 10.3390/ijms23020827. PMID: 35055012.
  • Soualah Z, Taly A, Crespin L, Saulais O, Henrion D, Legendre C, Tricoire-Leignel H, Legros C, Mattei C. (2021). GABAA Receptor Subunit Composition Drives Its Sensitivity to the Insecticide Fipronil. Front Neurosci. 15: 768466.
  • Park J, Taly A, Bourreau J, De Nardi F, Legendre C, Henrion D, Guérineau NC, Legros C, Mattei C, Tricoire-Leignel H. (2021). Partial Agonist Activity of Neonicotinoids on Rat Nicotinic Receptors: Consequences over Epinephrine Secretion and In Vivo Blood Pressure. Int J Mol Sci. 22(10):5106.
  • Arnich N, Abadie E, Amzil Z, Dechraoui Bottein MY, Comte K, Chaix E, Delcourt N, Hort V, Mattei C, Molgó J, Le Garrec R. (2021). Guidance Level for Brevetoxins in French Shellfish. Mar Drugs. 19(9): 520.
  • Arnich N, Abadie E, Delcourt N, Fessard V, Fremy JM, Hort V, Lagrange E, Maignien T, Molgó J, Peyrat MB, Vernoux JP, Mattei C. (2020). Health risk assessment related to pinnatoxins in French shellfish. Toxicon. 180: 1-10.
  •  Mattei C, Taly A, Saulais O, Henrion H, Guérineau NC, Verleye M, Legros C (2019). Involvement of the GABAA receptor alpha-subunit in the mode of action of etifoxine. Pharmacological Research. 145:104250.
  •  Frangieh J, Salma Y, Haddad K, Mattei C, Legros C, Fajloun Z, El Obeid D (2019). First Characterization of The Venom from Apis mellifera syriaca, A Honeybee from The Middle East Region. Toxins (Basel). 11(4). 191.
  •  Battault S, Meziat C, Nascimento A, Braud L, Gayrard S, Legros C, De Nardi F, Cazorla O, Drai J, Thireau J, Meyer G, and Reboul C (2018). Vascular endothelial function masks increased sympathetic vasopressor activity in rats with metabolic syndrome. Am J Physiol Heart Circ Physiol. H-00217-2017R1.
  •  De Nardi F, Lefort C, Bréard D, Richomme P, Legros C*, Guérineau NC* (2017). Monitoring the secretory behavior of the rat adrenal medulla by high-performance liquid chromatography-based catecholamine assay from slice supernatants. Front Endocrinol (Lausanne). 25;8:248.
  •  Khoury J, Dabbousya R, Riyad S, Antound S, Hleihele W, Legros C*, Fajloun Z* (2017). Evidence for in vitro antiophidian properties of aqueous buds extract of Eucalyptus against Montivipera bornmuelleri venom. J of Venom Res. 18;8:25-30.

 

Research

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